The Metagenomics workflow classifies bacteria from a metagenomic sample by
amplifying specific regions in 16S ribosomal RNA. Reads are classified using a database
of 16S rRNA data.

Metagenomics Pie Chart



Library Preparation:

Sample Preparation:

Bacterial Genomic DNA
Very clean (260/280 = 1.8 – 2.0)
Concentration:  At least 10ng/ul
Volume:  At least 20ul

Sample Submission:

All requests must be submitted through iLab.  See the links below for your particular institution.

UAMS Customers

Off Campus Customers

All Customers:
Please provide a gel documentation image of your samples, concentration, and 260/280 ratios. This is for QC purposes and we will not sequence your samples without this information.

Bring or ship your samples to:

Department of Microbiology/Immunology
UAMS, Biomedical Bld. II, Rm 321-2
4301 W. Markham
Little Rock, AR 72205-7199
(501) 686-6927

Data Delivery
Data will be delivered via Basespace, Illumina’s cloud computing service. Each customer is allowed 1 free Tb of space, including a variety of free apps for further analysis.

For publication purposes, please cite the following:

The study was supported in part by the Center for Microbial Pathogenesis and Host Inflammatory Responses grant P20GM103625 through the NIH National Institute of General Medical Sciences Centers of Biomedical Research Excellence. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.