Sorting and Staining Buffers

2% Paraformaldehyde

2 grams paraformaldehyde / 100 ml of 1X PBS

Heat at 55 degrees for 1 hour or microwave for 10 to 30 seconds until it goes into solution.

Place on ice until use.

To fix samples:  Centerfuge samples, remove supernatants, vortex pellet, resuspend cells in 0.5 ml of 2% paraformaldehyde/sample.  Store samples on ice and discard unused portion of paraformaldehyde.

Note:  2% paraformaldehyde should be made fresh for each staining assay use.