Postdoctoral Fellow
Ph.D.:  University of Copenhagen, Denmark
Mentor:  Chia Lee, Ph.D.
Phone: (501) 526-7690
Email

 

Research Description

I received my Ph.D. in molecular bacteriology and infection from the University of Copenhagen, Denmark, in 2015. After that, I joined Dr. Chia Y. Leeˈs laboratory in April 2016 where I am currently involved in a project aimed at understanding virulence regulation in Staphylococcus aureus, an important human pathogen. The pathogenicity S. aureus is mainly due to production of plethora of secreted and cell wall-associated virulence factors that are thought to be coordinately regulated depending on environmental cues and infection stages. To further understand virulence regulation in this pathogen, I am analyzing the possibility of post-translational regulation of Agr by ClpC, an ATPase chaperone. This is based a recent finding in our lab that showed AgrA, the response regulator of the Agr system, is one of the substrates of ClpC. I am now working to test the hypothesis of ClpC-AgrA interaction by using protein-protein interaction principles, and elucidate how such interaction affects the Agr system. Many studies about virulence gene regulation in S. aureus are conducted in vitro condition and a few is known regarding virulence regulation under in vivo condition.  In vitro studies shows that hla is activated by Sae, MgrA and CcpA. In the second part of the project, I am working to elucidate the role of these regulators on α-toxin production in disease relevant animal by using rat infective endocarditis and rat subcutaneous abscess models.

Publications

Gudeta DD, Bortolaia V, Jayol A, Poirel L, Nordmann P, Guardabassi L. Chromobacterium spp. harbour Ambler class A ß-lactamases showing high identity with KPC. J Antimicrob Chemother. 2016 Jun; 71(6):1493-6. PMID: 26892778. View in: PubMed

Gudeta DD, Bortolaia V, Amos G, Wellington EM, Brandt KK, Poirel L, Nielsen JB, Westh H, Guardabassi L. The Soil Microbiota Harbors a Diversity of Carbapenem-Hydrolyzing ß-Lactamases of Potential Clinical Relevance. Antimicrob Agents Chemother. 2016 Jan; 60(1):151-60. PMID: 26482314.View in: PubMed

Gudeta DD, Moodley A, Bortolaia V, Guardabassi L. vanO, a new glycopeptide resistance operon in environmental Rhodococcus equi isolates. Antimicrob Agents Chemother. 2014; 58(3):1768-70. PMID: 24342631.View in: PubMed

Frees D, Andersen JH, Hemmingsen L, Koskenniemi K, Bæk KT, Muhammed MK, Gudeta DD, Nyman TA, Sukura A, Varmanen P, Savijoki K. New insights into Staphylococcus aureus stress tolerance and virulence regulation from an analysis of the role of the ClpP protease in the strains Newman, COL, and SA564. J Proteome Res. 2012 Jan 1; 11(1):95-108. PMID: 22112206.View in: PubMed

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