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Home » Research Cores » Flow Cytometry Core Facility » Instruments » FacsAria
FacsAria


The FACSAria has 3 lasers and is able to detect up to 9 colors at one time as well as sort up to 4 populations at once. The FACS Aria is used for analysis of more than 4 colors and cell sorting, its primary function being cell sorting. The FACS Aria is also used when a fluorochrome is required that cannot be detected by the FACS Calibur.

Lasers
488-nm Coherent® SapphireTM solid state
633-nm JDS UniphaseTM HeNe air-cooled
407-nm Point Source Violet solid state
All lasers are Class IIIb.

Excitation Laser

Detection Filter

Detector

Example

488 nm (blue)

488/10 (483-493 nm)

FSC Diode

FSC only

 

780/60 (750-810 nm)

PMT A

PE-Cy7

 

695/40 (675-715 nm)

PMT B

PerCP Cy5.5

 

610/20 (600-620 nm)

PMT C

PE-Texas Red

 

576/26 (563-589 nm)

PMT D

PE

 

530/30 (515-545 nm)

PMT E

FITC

 

488/10 (483-493 nm)

PMT F

SSC only

 

 

 

 

633 nm (red)

780/60 (750-810 nm)

PMT A

APC-Cy7

  720/40 (700-740 nm)

PMT B

AF 700

 

660/20 (650-670 nm)

PMT C

APC

 

 

 

 

407 nm (violet)

 450/40 (430-470 nm)

PMT A

DAPI 

Analysis

Analysis and acquisition on the FACS Aria is done in the FACS Diva software. Using FACS Diva, data may be analyzed on dot plots and histograms with various gates.

Data Delivery

Please bring a USB drive or blank CD to save your data files. The Core Facility does not store or archive data for longer than about 6 months so it is your responsibility to get the data files and save them for future use.